Apex 2X GREEN Master Mixes are single-tube 2x reagents including all components necessary to perform DNA-binding dye based real-time DNA amplification. Just add your primers and DNA. Apex qPCR 2x Master Mixes are available with high, low or without ROX™ for optimal performance on most of the commonly used real-time PCR instruments. The qPCR Master Mixes promote high specificity and low background by using Apex Hot Start DNA polymerase, a modified Taq DNA polymerase with hot start capabilities. The unopened product is stable at -20 °C for 2 years. Store the qPCR 2X Master Mix at +4 °C after thawing. Once thawed, full activity is guaranteed for 3 months.
Applications include genomic and cDNA, absolute and relative quantitation, presence/absence experiments, SNP analysis, genotyping, and pathogen detection. Detection limit: approximately 1 copy. Quantitation limit: approximately 24 copies (0.08 ng of human gDNA, correlating to 12 diploid genomes, with 2 gene copies per diploid genome)