High Resolution Melt (HRM) analysis is a powerful post-PCR analysis method used to detect and identify mutations, polymorphisms and epigenetic differences in double-stranded DNA samples. HRM detection is a cost-effective solution for analyzing sequence variations in a variety of applications and offers several advantages over other technologies. Samples are analyzed by looking at the difference in melt curve shapes and SNA melting temperature to identify sequence variations between samples.
qPCRBIO HRM Mix uses the novel SyGreen 2 intercalating dye. SyGreen 2 does not inhibit PCR and is a saturating, 3rd generation dye essential for accurate SNP genotyping. Unlike first generation dyes, SyGreen 2 can be used at high concentrations and does not have a different affinity for AT or GC rich sequences. Combining the latest advancements in polymerase technology and advanced buffer chemistry we offer market leading performance with minimal or no optimisation. qPCRBIO HRM Mix delivers accurate detection of subtle sequence variations including class I to class IV mutations and CpG methylation differences. Many modern real-time PCR instruments have a fast cycling mode. The same developments that improve the sensitivity and consistency of qPCRBIO HRM Mix in standard cycling conditions also allow for industry leading performance in fast and ultra fast cycling conditions.